abstract：BACKGROUND:ADB-PINACA and its 5-fluoropentyl analog 5F-ADB-PINACA are among the most potent synthetic cannabinoids tested to date, with several severe intoxication cases. ADB-PINACA and 5F-ADB-PINACA have a different legal status, depending on the country. Synthetic cannabinoid metabolites predominate in urine, making ...

journal_title：Clinical chemistry

authors： Carlier J,Diao X,Scheidweiler KB,Huestis MA

更新日期：2017-05-01 00:00:00

abstract：BACKGROUND:Recent studies have suggested that single-stranded DNA (ssDNA) library preparation can enrich short DNA species from the plasma of healthy individuals, cancer patients, and transplant recipients. Based on previous observations that fetal DNA molecules in the maternal plasma are shorter than maternal DNA mole...

journal_title：Clinical chemistry

authors： Vong JSL,Tsang JCH,Jiang P,Lee WS,Leung TY,Chan KCA,Chiu RWK,Lo YMD

更新日期：2017-05-01 00:00:00

abstract：BACKGROUND:Pompe disease (PD) is the first lysosomal storage disorder to be added to the Recommended Uniform Screening Panel for newborn screening. This condition has a broad phenotypic spectrum, ranging from an infantile form (IOPD), with severe morbidity and mortality in infancy, to a late-onset form (LOPD) with vari...

journal_title：Clinical chemistry

authors： Lin N,Huang J,Violante S,Orsini JJ,Caggana M,Hughes EE,Stevens C,DiAntonio L,Chieh Liao H,Hong X,Ghomashchi F,Babu Kumar A,Zhou H,Kornreich R,Wasserstein M,Gelb MH,Yu C

更新日期：2017-04-01 00:00:00

abstract：BACKGROUND:Identifying novel risk markers in cardiovascular patients remains a research priority. Longer follow-up generally is considered favorable in such studies, but associations of interest may become attenuated with increasing follow-up. This issue has not been adequately addressed in the context of patient cohor...

journal_title：Clinical chemistry

authors： Breitling LP,Mons U,Hahmann H,Koenig W,Rothenbacher D,Brenner H

更新日期：2017-03-01 00:00:00

abstract：BACKGROUND:Cardiac troponin T (cTnT) is the preferred biomarker for the diagnosis of acute myocardial infarction (AMI). It has been suggested that cTnT is present predominantly in fragmented forms in human serum following AMI. In this study, we have used a targeted mass spectrometry assay and epitope mapping using West...

journal_title：Clinical chemistry

authors： Streng AS,de Boer D,van Doorn WP,Bouwman FG,Mariman EC,Bekers O,van Dieijen-Visser MP,Wodzig WK

更新日期：2017-02-01 00:00:00

abstract：BACKGROUND:Increased cardiac troponin concentrations in acute coronary syndrome (ACS) identify patients with ongoing cardiomyocyte necrosis who are at increased risk. However, with the use of more precise assays, cardiac troponin increases are commonly noted in other cardiovascular conditions as well. This has generate...

journal_title：Clinical chemistry

authors： Eggers KM,Lindahl B

更新日期：2017-01-01 00:00:00

abstract：BACKGROUND:Soluble CD146 (sCD146), is an endothelial marker with similar diagnostic power as natriuretic peptides in decompensated heart failure (HF). While natriuretic peptides are released by the failing heart, sCD146 may be released by veins in response to stretch induced by systemic congestion in HF. This study inv...

journal_title：Clinical chemistry

authors： Arrigo M,Truong QA,Onat D,Szymonifka J,Gayat E,Tolppanen H,Sadoune M,Demmer RT,Wong KY,Launay JM,Samuel JL,Cohen-Solal A,Januzzi JL Jr,Singh JP,Colombo PC,Mebazaa A

更新日期：2017-01-01 00:00:00

abstract：BACKGROUND:Evidence suggests that systemic inflammation may adversely impact HDL function. In this study we sought to evaluate the independent and incremental predictive performance of GlycA-a novel serum inflammatory biomarker that is an aggregate measure of enzymatically glycosylated acute phase proteins-and HDL subc...

journal_title：Clinical chemistry

authors： McGarrah RW,Kelly JP,Craig DM,Haynes C,Jessee RC,Huffman KM,Kraus WE,Shah SH

更新日期：2017-01-01 00:00:00

abstract：BACKGROUND:Recent studies have suggested that glial fibrillary acidic protein (GFAP) serum concentrations distinguish between intracerebral hemorrhage (ICH) and ischemic stroke (IS) shortly after symptom onset. In this prospective multicenter trial we validated GFAP in an independent patient cohort and assessed the qua...

journal_title：Clinical chemistry

authors： Luger S,Witsch J,Dietz A,Hamann GF,Minnerup J,Schneider H,Sitzer M,Wartenberg KE,Niessner M,Foerch C,BE FAST II and the IGNITE Study Groups.

更新日期：2017-01-01 00:00:00

abstract：BACKGROUND:An assay using a mouse antisialyl Lewis X (sLeX) antibody (CSLEX-1) is used clinically for screening and monitoring patients with breast cancer in Japan. However, the IgM isoform of CSLEX-1 is not preferred for the assay because the bulkiness of IgM generally causes poor accessibility to the antigen. To solv...

journal_title：Clinical chemistry

authors： Yamashita J,Kobayashi I,Tatematsu K,Sezutsu H,Noda K,Ishihara H

更新日期：2016-11-01 00:00:00

abstract：BACKGROUND:Galectin-3 has emerged as a potential useful novel biomarker for heart failure and cardiovascular disease (CVD). However, it remains unclear whether galectin-3 is associated with recurrent cardiovascular events during long-term follow-up of patients with stable coronary heart disease (CHD) after adjustment f...

journal_title：Clinical chemistry

authors： Jansen H,Koenig W,Jaensch A,Mons U,Breitling LP,Scharnagl H,Stojakovic T,Schunkert H,Brenner H,Rothenbacher D

更新日期：2016-10-01 00:00:00

abstract：BACKGROUND:Although it is being increasingly applied, blood collection for drug testing in sport presents some logistic issues that complicate full applicability on a large scale. The use of dried blood spots (DBS) could benefit compliant blood testing considerably owing to its simplicity, minimal invasiveness, analyte...

journal_title：Clinical chemistry

authors： Reverter-Branchat G,Bosch J,Vall J,Farré M,Papaseit E,Pichini S,Segura J

更新日期：2016-10-01 00:00:00

abstract：BACKGROUND:Absolute plasma hepcidin concentrations measured by various procedures differ substantially, complicating interpretation of results and rendering reference intervals method dependent. We investigated the degree of equivalence achievable by harmonization and the identification of a commutable secondary refere...

journal_title：Clinical chemistry

authors： van der Vorm LN,Hendriks JC,Laarakkers CM,Klaver S,Armitage AE,Bamberg A,Geurts-Moespot AJ,Girelli D,Herkert M,Itkonen O,Konrad RJ,Tomosugi N,Westerman M,Bansal SS,Campostrini N,Drakesmith H,Fillet M,Olbina G,Pasricha

更新日期：2016-07-01 00:00:00

abstract：BACKGROUND:First introduced in 2006, recombinase polymerase amplification (RPA) has stirred great interest, as evidenced by 75 publications as of October 2015, with 56 of them just in the last 2 years. The widespread adoption of this isothermal molecular tool in many diagnostic fields represents an affordable (approxim...

journal_title：Clinical chemistry

authors： Daher RK,Stewart G,Boissinot M,Bergeron MG

更新日期：2016-07-01 00:00:00

abstract：BACKGROUND:Several compounds in the choline oxidation pathway are associated with insulin resistance and prevalent diabetes; however, prospective data are scarce.We explored the relationships between systemic and urinary choline-related metabolites and incident type 2 diabetes in an observational prospective study amon...

journal_title：Clinical chemistry

authors： Svingen GF,Schartum-Hansen H,Pedersen ER,Ueland PM,Tell GS,Mellgren G,Njølstad PR,Seifert R,Strand E,Karlsson T,Nygård O

更新日期：2016-05-01 00:00:00

abstract：BACKGROUND:FMR1 full mutations (FMs) (CGG expansion >200) in males mosaic for a normal (<45 CGG) or gray-zone (GZ) (45-54 CGG) allele can be missed with the standard 2-step fragile X syndrome (FXS) testing protocols, largely because the first-line PCR tests showing a normal or GZ allele are not reflexed to the second-l...

journal_title：Clinical chemistry

authors： Aliaga SM,Slater HR,Francis D,Du Sart D,Li X,Amor DJ,Alliende AM,Santa Maria L,Faundes V,Morales P,Trigo C,Salas I,Curotto B,Godler DE

更新日期：2016-02-01 00:00:00

abstract：BACKGROUND:MALDI-TOF mass spectrometry (MS) is set to make inroads into clinical chemistry because it offers advantages over other analytical platforms. These advantages include low acquisition and operating costs, ease of use, ruggedness, and high throughput. When coupled with innovative front-end strategies and appli...

journal_title：Clinical chemistry

authors： Duncan MW,Nedelkov D,Walsh R,Hattan SJ

更新日期：2016-01-01 00:00:00

abstract：BACKGROUND:Recent studies show that the haptoglobin phenotype in individuals with diabetes mellitus is an important factor for predicting the risk of myocardial infarction, cardiovascular death, and stroke. Current methods for haptoglobin phenotyping include PCR and gel electrophoresis. A need exists for a reliable met...

journal_title：Clinical chemistry

authors： Pompach P,Nováková J,Kavan D,Benada O,Růžička V,Volný M,Novák P

更新日期：2016-01-01 00:00:00

abstract：INTRODUCTION:Parathyroid hormone-related peptide (PTHrP) is involved in activating pathways, allowing tumor cells to form bone metastases. Measurement of PTHrP is used for the diagnosis and clinical management of patients suspected of hypercalcemia of malignancy. We developed an LC-MS/MS method for measuring PTHrP, est...

journal_title：Clinical chemistry

authors： Kushnir MM,Rockwood AL,Strathmann FG,Frank EL,Straseski JA,Meikle AW

更新日期：2016-01-01 00:00:00

abstract：BACKGROUND:Different technologies, such as quantitative real-time PCR or microarrays, have been developed to measure microRNA (miRNA) expression levels. Quantification of miRNA transcripts implicates data normalization using endogenous and exogenous reference genes for data correction. However, there is no consensus ab...

journal_title：Clinical chemistry

authors： Schwarzenbach H,da Silva AM,Calin G,Pantel K

更新日期：2015-11-01 00:00:00

abstract：BACKGROUND:The association between increases in cardiac troponin and adverse cardiac outcomes is well established. There is a growing interest in exploring routine cardiac troponin monitoring as a potential early indicator of adverse heart health trends. Prognostic use of cardiac troponin measurements requires an assay...

journal_title：Clinical chemistry

authors： Jarolim P,Patel PP,Conrad MJ,Chang L,Melenovsky V,Wilson DH

更新日期：2015-10-01 00:00:00

abstract：BACKGROUND:We used a difference in bias approach to evaluate the commutability of 4 frozen serum pools for 8 direct methods for measurement of HDL and LDL cholesterol (HDLC and LDLC). METHODS:Freshly collected nonfrozen sera from 138 diseased and 37 nondiseased patients and 4 frozen pools from the CDC Lipid Standardiz...

journal_title：Clinical chemistry

authors： Korzun WJ,Nilsson G,Bachmann LM,Myers GL,Sakurabayashi I,Nakajima K,Nakamura M,Shamburek RD,Remaley AT,Miller WG

更新日期：2015-08-01 00:00:00

abstract：BACKGROUND:Noninvasive prenatal diagnosis of monogenic disorders using maternal plasma and targeted massively parallel sequencing is being investigated actively. We previously demonstrated that comprehensive genetic diagnosis of a Duchenne muscular dystrophy (DMD) patient is feasible using a single targeted sequencing ...

journal_title：Clinical chemistry

authors： Yoo SK,Lim BC,Byeun J,Hwang H,Kim KJ,Hwang YS,Lee J,Park JS,Lee YS,Namkung J,Park J,Lee S,Shin JY,Seo JS,Kim JI,Chae JH

更新日期：2015-06-01 00:00:00

abstract：BACKGROUND:24,25-Dihydroxyvitamin D [24,25(OH)2D] in serum may be both a nuisance and nutritionally valuable. METHODS:We investigated the impact of 24,25(OH)2D3 on the performance of commercially available immunoassays for serum total 25-hydroxyvitamin D [25(OH)D] using (a) serum from a nationally representative sampl...

journal_title：Clinical chemistry

authors： Cashman KD,Hayes A,Galvin K,Merkel J,Jones G,Kaufmann M,Hoofnagle AN,Carter GD,Durazo-Arvizu RA,Sempos CT

更新日期：2015-04-01 00:00:00

abstract：BACKGROUND:We investigated agreement between self-reported prenatal alcohol exposure (PAE) and objective meconium alcohol markers to determine the optimal meconium marker and threshold for identifying PAE. METHODS:Meconium fatty acid ethyl esters (FAEE), ethyl glucuronide (EtG), and ethyl sulfate (EtS) were quantified...

journal_title：Clinical chemistry

authors： Himes SK,Dukes KA,Tripp T,Petersen JM,Raffo C,Burd L,Odendaal H,Elliott AJ,Hereld D,Signore C,Willinger M,Huestis MA,Prenatal Alcohol in SIDS and Stillbirth (PASS) Network.

更新日期：2015-03-01 00:00:00

abstract：BACKGROUND:Next generation sequencing (NGS)-based assays continue to redefine the field of genetic testing. Owing to the complexity of the data, bioinformatics has become a necessary component in any laboratory implementing a clinical NGS test. CONTENT:The computational components of an NGS-based work flow can be conc...

journal_title：Clinical chemistry

authors： Oliver GR,Hart SN,Klee EW

更新日期：2015-01-01 00:00:00

abstract：BACKGROUND:The diagnosis of 22q11 deletion syndrome (22q11DS) is often delayed or missed due to the wide spectrum of clinical involvement ranging from mild to severe, often life-threatening conditions. A delayed diagnosis can lead to life-long health issues that could be ameliorated with early intervention and treatmen...

journal_title：Clinical chemistry

authors： Pretto D,Maar D,Yrigollen CM,Regan J,Tassone F

更新日期：2015-01-01 00:00:00

abstract：BACKGROUND:First introduced into clinical microbiology laboratories in Europe, MALDI-TOF MS is being rapidly embraced by laboratories around the globe. Although it has multiple applications, its widespread adoption in clinical microbiology relates to its use as an inexpensive, easy, fast, and accurate method for identi...

journal_title：Clinical chemistry

authors： Patel R

更新日期：2015-01-01 00:00:00

abstract：BACKGROUND:Genomic instability resulting in copy number variation is a hallmark of malignant transformation and may be identified through massive parallel sequencing. Tumor-specific cell free DNA (cfDNA) present in serum and plasma provides a real-time, easily accessible surrogate. METHODS:DNA was extracted from serum...

journal_title：Clinical chemistry

authors： Schütz E,Akbari MR,Beck J,Urnovitz H,Zhang WW,Bornemann-Kolatzki K,Mitchell WM,Nam RK,Narod SA

更新日期：2015-01-01 00:00:00

abstract：BACKGROUND:Late enhancement MRI (LE-MRI) and cardiac troponin I (cTnI) are sensitive methods to detect subclinical myocardial injury. We sought to investigate the relation between plasma concentrations of cTnI measured with a high-sensitivity assay (hs-cTnI) and the development of unrecognized myocardial infarctions (U...

journal_title：Clinical chemistry

authors： Barbier CE,Themudo R,Bjerner T,Johansson L,Lindahl B,Venge P,Lind L,Ahlström H

更新日期：2014-10-01 00:00:00

abstract：BACKGROUND:Microsatellite instability (MSI) is a useful phenotype in cancer diagnosis and prognosis. Nevertheless, methods to detect MSI status from next generation DNA sequencing (NGS) data are underdeveloped. METHODS:We developed an approach to detect the MSI phenotype using NGS (mSINGS). The method was used to eval...

journal_title：Clinical chemistry

authors： Salipante SJ,Scroggins SM,Hampel HL,Turner EH,Pritchard CC

更新日期：2014-09-01 00:00:00

abstract：BACKGROUND:Quantification of integrated proviral HIV DNA by repetitive-sampling Alu-HIV PCR is a candidate virological tool to monitor the HIV reservoir in patients. However, the experimental procedures and data analysis of the assay are complex and hinder its widespread use. Here, we provide an improved and simplified...

journal_title：Clinical chemistry

authors： De Spiegelaere W,Malatinkova E,Lynch L,Van Nieuwerburgh F,Messiaen P,O'Doherty U,Vandekerckhove L

更新日期：2014-06-01 00:00:00

abstract：BACKGROUND:Human herpesvirus 6 (HHV-6) latently infects a majority of adults. In about 1% of the population HHV-6 exists in a chromosomally integrated form (ciHHV-6) that resides in every somatic and germ cell and can be transmitted through the germ line. Patients with ciHHV-6 have been misdiagnosed and unnecessarily t...

journal_title：Clinical chemistry

authors： Sedlak RH,Cook L,Huang ML,Magaret A,Zerr DM,Boeckh M,Jerome KR

更新日期：2014-05-01 00:00:00

abstract：BACKGROUND:The goal of this study was to establish a plasma microRNA profile by use of next-generation sequencing that could aid in assessment of patient prognosis in nasopharyngeal carcinoma (NPC). METHODS:Two panels of NPC patients and healthy controls (HCs) were recruited for this study. We used deep sequencing to ...

journal_title：Clinical chemistry

authors： Wang HY,Yan LX,Shao Q,Fu S,Zhang ZC,Ye W,Zeng YX,Shao JY

更新日期：2014-05-01 00:00:00

abstract：BACKGROUND:Cerebrospinal fluid (CSF) tau is a common biomarker for Alzheimer disease (AD). Measurements of tau have historically been performed using immunoassays. Given the molecular diversity of tau in CSF, the selectivity of these immunoassays has often been questioned. Therefore, we aimed to develop an analytically...

journal_title：Clinical chemistry

authors： McAvoy T,Lassman ME,Spellman DS,Ke Z,Howell BJ,Wong O,Zhu L,Tanen M,Struyk A,Laterza OF

更新日期：2014-04-01 00:00:00

abstract：BACKGROUND:Group B streptococcal infections are the leading cause of sepsis and meningitis in newborns. A rapid and reliable method for the detection of this pathogen at the time of delivery is needed for the early treatment of neonates. Isothermal amplification techniques such as recombinase polymerase amplification h...

journal_title：Clinical chemistry

authors： Daher RK,Stewart G,Boissinot M,Bergeron MG

更新日期：2014-04-01 00:00:00

abstract：BACKGROUND:The B-type natriuretic peptides (BNP and N-terminal pro-BNP) are secreted by the heart and, in the case of BNP, serve to maintain circulatory homeostasis through renal and vascular actions and oppose many effects of the renin-angiotensin system. Recent evidence suggests that in patients with severe heart fai...

journal_title：Clinical chemistry

authors： Mahagamasekera PG,Ruygrok PN,Palmer SC,Richards AM,Ansell GS,Nicholls MG,Pemberton CJ,Lewis LK,Yandle TG

更新日期：2014-03-01 00:00:00

abstract：BACKGROUND:There is extended urinary excretion of Δ(9)-tetrahydrocannabinol (THC), 11-hydroxy-THC (11-OH-THC), and 11-nor-9-carboxy-THC (THCCOOH) in abstinent frequent cannabis smokers. We characterized THC, 11-OH-THC, THCCOOH, cannabidiol, cannabinol, THC-glucuronide, and THCCOOH-glucuronide disposition in urine of fr...

journal_title：Clinical chemistry

authors： Desrosiers NA,Lee D,Concheiro-Guisan M,Scheidweiler KB,Gorelick DA,Huestis MA

更新日期：2014-02-01 00:00:00

abstract：BACKGROUND:Blood concentrations of the calcineurin inhibitors (CNIs) cyclosporine and tacrolimus are currently measured to monitor immunosuppression in transplant patients. The measurement of calcineurin (CN) phosphatase activity has been proposed as a complementary pharmacodynamic approach. However, determining CN act...

journal_title：Clinical chemistry

authors： Carr L,Gagez AL,Essig M,Sauvage FL,Marquet P,Gastinel LN

更新日期：2014-02-01 00:00:00

abstract：BACKGROUND:The fibroblast growth factor (FGF) and FGF receptor (FGFR) axis plays a critical role in tumorigenesis, but little is known of its influence in ovarian cancer. We sought to determine the association of genetic variants in the FGF pathway with risk, therapeutic response, and survival of patients with ovarian ...

journal_title：Clinical chemistry

authors： Meng QH,Xu E,Hildebrandt MA,Liang D,Lu K,Ye Y,Wagar EA,Wu X

更新日期：2014-01-01 00:00:00